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Reliable diseases such as diabetes mellitus,8 hypertension and reno- quantitative indices of free radical—induced modification of vascular disease,9 hyperlipidemia,10 obesity,11 and cigarette DNA, proteins, and lipids in vivo have emerged only recent- smoking.

Morrow et al5 have identified isoprostanes sure, elevated total cholesterol, LDL cholesterol, HDL cho- as end products of lipid peroxidation. Isoprostanes are chem- lesterol, diabetes mellitus, obesity, cigarette smoking, and Received September 24, ; revision received November 7, ; accepted November 18, Schwedhelm was responsible for the methodology of isoprostane measurement, interpretation of data, and writing the report.

Bartling and H. Lenzen were responsible for patient recruiting and organization of urine and plasma analysis. Tsikas and F. Gutzki were responsible for the isoprostane measurement. Mass did data analysis and interpretation. Berger was responsible for statistical analysis and interpretation of data. E-mail schwedhelm uke. Validity of the method was proven ships between these risks have elucidated their additive as reported previously.

The relative magnitude at which nine concentration to account for differences in renal excretory markers of oxidative stress such as isoprostanes contribute to function.

Plasma concentration of oxidized LDL cholesterol was these risk factors is as yet unknown. In addition, the extent to determined with a commercially available immunoassay Mercodia which oxidative stress contributes independently to hyperten- SA. Finally, increasing oxidative stress from different lometer with polystyrene microbeads coated with monoclonal mouse antibodies.

Ninety-three patients with a confirmed diagnosis of CHD were included and frequency-matched by Calculations and Statistical Methods All data were tested for normal distribution with the Kolmogorov- age and sex with control subjects controls from the general Smirnov test.

Because explorative data analysis was performed, no correction of the type I error was made. Differences between groups further biochemical parameters. Comparisons between 2 subgroups Methods were performed with the Mann-Whitney U test 2-sided. Continu- Patients and Study Protocol ous variables were split into tertiles for univariate and multivariate Patients between 35 and 80 years old with established coronary analysis.

Categorized biochemical characteristics body mass index, artery disease were recruited for this study. Biochemical characteristics were tigraphy, documentation of coronary stenosis by coronary angiogra- included stepwise in one multivariate model and risk factors of CHD, phy. Estimates of general population via local newspaper advertisement.

For statistical for coronary artery disease. Patients or controls suffering from any analyses, SPSS version Characteristics of patients and from the study. We assessed eligibility, obtained informed consent, controls are given in Table 1. Total cholesterol, LDL choles- and included 93 patients and 93 controls in the study. The study protocol was approved by the local Institutional Review Board for terol, HDL cholesterol, and triglyceride levels were lower in Studies in Humans.

Systolic blood pressure and body All patients and controls were invited to the study center in the mass index were higher in the CHD group. Oxidative stress morning. The patients were asked for their previous medical history and inflammation were evaluated by oxidized LDL, isopros- and the presence of cardiovascular risk factors by use of a standard- tanes, and high-sensitive CRP, respectively. CRP was more ized questionnaire. Blood samples were centrifuged factors for both groups is given in Table 2.

Diurnal variation of cholesterolemia, or hypertension. Patients were also more creatinine-indexed isoprostane excretion was assessed in 3 subjects. Treatment with lipid-lowering remained strong and independent predictors of CHD. There was no correlation between the 2 isoprostanes and oxidized LDL.

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